Dr. Rafael Gutiérrez Aguilar
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Investigador CINVESTAV 3D |
Línea de Investigación:
Plasticidad Sináptica y Epileptogénesis. Con técnicas electrofisiológicas, inmunocitoquímicas y de biología molecular estudiamos la expresión del fenotipo dual glutamatérgico / GABAérgico en las células granulares del hipocampo. Nosotros descubrimos que estas células expresan todos los marcadores de ambos fenotipos y la expresión del fenotipo GABAérgico está regulada por el desarrollo y, en el adulto, de manera dependiente de actividad, especialmente por crisis convulsivas.
Temas de Investigación:
- Plasticidad sináptica
- Epileptogénesis
- Modulación presináptica
Metodología:
- Usamos técnicas electrofisiológicas,
inmunocitoquímicas y de biología molecular
Publicaciones representativas:
- Gutiérrez R. Seizures induce simultaneous GABAergic and glutamatergic
neurotransmission in the dentate gyrus – CA3 system. J. Neurophysiol.
84:3088-3090, 2000. Pdf
- Gutiérrez R. and Heinemann U. Kindling induces transient fast
inhibition in the dentate gyrus – CA3 projection. Eur. J. Neuroscience
13, 1371-1379, 2001. Pdf
- Gutiérrez R., Leff P., Romo-Parra, H., Acevedo, R., Antón
B. Orphanin-FQ/nociceptin inhibits kindling epileptogenesis and enhances
hippocampal feed-forward inhibition. Neuroscience. 105: 325-333, 2001. Pdf
- M. Ramírez, R. Gutiérrez R. Activity-dependent expression
of GAD67 in the granule cells of the rat hippocampus. Brain Res. 917(2):
139-146, 2001. Pdf
- Lamas M., Gómez-Lira G. and Gutiérrez R. Vesicular GABA
transporter mRNA expression in the dentate gyrus and in mossy fiber synaptosomes.
Mol. Brain Res. 93(2):209-214, 2001. Pdf
- Gutiérrez R. Activity-dependent expression of simultaneous glutamatergic
and GABAergic neurotransmission from the mossy fibers in vitro. J. Neurophysiol.
87: 2562-2570, 2002. Pdf
- Gómez-Lira G., Trillo E., Ramírez M., Asai M. Sitges M.,
Gutiérrez R. The expression of GABA in mossy fiber synaptosomes coincides
with the seizure-induced expression of GABAergic transmission in the mossy
fiber synapse. Exp. Neurol. 177: 276-283, 2002. Pdf
- Romo-Parra, H., Vivar C., Maqueda J., Morales M.A., Gutiérrez,
R. Activity-dependent induction of multitransmitter signaling onto pyramidal
cells and interneurons of area CA3 of the rat hippocampus. J. Neurophysiol.
89: 3155-3167, 2003. Pdf
- Gutiérrez,R., Romo-Parra, H., Maqueda, J., Vivar, C., Ramírez,
M., Morales M.A., Lamas, M. Plasticity of the GABAergic phenotype of the
“glutamatergic” granule cells of the rat dentate gyrus. J. Neurosci.,
23(13): 5594-5598, 2003. Pdf
- Maqueda, J., Ramírez, M., Lamas, M., Gutiérrez,R. Glutamic
acid decarboxylase (GAD)67, but not GAD65, is constitutively expressed during
development and transiently overexpressed by activity in the mossy fibers.
Neurosci. Lett. 353: 69-71, 2003. Pdf
- Gutiérrez, R.The GABAergic phenotype of the “glutamatergic”
granule cells of the dentate gyrus. Progress in Neurobiol. 71: 337-358,
2003. Pdf
- Romo-Parra, H., Aceves, J., Gutiérrez, R. Tonic modulation of inhibition
by dopamine D4 receptors in the rat hippocampus. Hippocampus 15:254-259,
2005. Pdf
- Vivar, C., Gutiérrez, R. Blockade of the membranal GABA transporter
potentiates GABAergic responses evoked in pyramidal cells by mossy fiber
activation after seizures. Hippocampus. 15:281-284, 2005. Pdf
- Gutiérrez, R. The dual glutamatergic-GABAergic phenotype of the
hippocampal granule cells. Trends in Neurosci. 28: 297-303, 2005. Pdf
- Gómez-Lira, G., Lamas, M., Romo-Parra, H., Gutiérrez, R.
Programmed and induced phenotype of the hippocampal granule cells. J. Neurosci. 25:6939-6946, 2005
Pdf
- Treviño, M., Gutiérrez, R. The GABAaergic projection of the dentate gyrus to hippocampal area CA3 of the rat: pre- and postsynaptic actions after seizures. J. Physiol. 567: 939-949, 2005.
Pdf
- Gutiérrez, R., Heinemman Uwe. Co-Existence of GABA and Glu in the Hippocampal Granule Cells: Implications for Epilepsy. Current Topics in Medicinal Chemistry, 6:975-978, 2006
Pdf
- Treviño, M., Vivar, C., Gutiérrez, R. β/γ Oscillatory activity in the CA3 hippocampal area is depressed by aberrant GABAergic transmission from the dentate gyrus after seizures. J. Neurosci. 27: 251-259, 2007.
Pdf
- Trudeau, L.E. and Gutiérrez, R. On co-transmission and neurotransmitter phenotype plasticity. Molecular Interventions 7: 138-146, 2007. Pdf
- Treviño, M., Gutiérrez, R. Las bases celulares de las oscilaciones neuronales. Salud Mental 30(2), 2007. ** Pdf
- Jaffe DB, Gutiérrez R. Mossy fiber synaptic transmission: communication from the dentate gyrus to area CA3. Prog Brain Res.163:109-805, 2007. Pdf
- Romo-Parra, H., Treviño, M., Heinemann, U., Gutiérrez, R. GABA Actions in Hippocampal Area CA3 During Postnatal Development: Differential Shift From Depolarizing to Hyperpolarizing in Somatic and Dendritic Compartments. J Neurophysiol 99: 1523-1534, 2008. Pdf
- Gutiérrez, R. Ex uno plures: Out of one, many. Co-release of classical neurotransmitters. In Gutiérrez, R. Co-existence and Co-release of Classical Neurotransmitters. Ex uno plures. Springer Verlag. 2008, pp. 15-22. Pdf
- Gutiérrez, R. The dual glutamatergic/GABAergic phenotype of the hippocampal granule cells In Gutiérrez, R. Co-existence and Co-release of Classical Neurotransmitters. Ex uno plures. Springer Verlag. 2008, pp. 181-202. Pdf
- Gutiérrez, R., Arias, J.A. E pluribus unum: Out of many, one. Post-synaptic integration of co-transmitters. In Gutiérrez, R. Co-existence and Co-release of Classical Neurotransmitters. Ex uno plures. Springer Verlag. 2008, pp. 263-272. Pdf
- Gutiérrez R Phenotypic Plasticity of the Granule Cells and its Relation to Epilepsy. In: Philip A. Schwartzkroin, editor Encyclopedia of Basic Epilepsy Research, Vol 1. Oxford: Academic Press; 2009. pp. 463-472. Pdf
- Libro: Co-Existence and Co-Release of Classical Neurotransmitters
- Zander, J.F., Münster-Wandowski, A., Brunk, I., Pahner, I., Gómez-Lira, G., Heinemann, U., Gutiérrez, R., Laube, G., Ahnert-Hilger, G. Synaptic and vesicular co-existence of VGLUT and VGAT in selected excitatory and inhibitory synapses,. J.Neurosci. 30:7634 –7645, 2010. Pdf
Profesor Titular
Departamento de Fisiología Biofísica y Neurociencias
Centro de Investigación y Estudios Avanzados del IPN,
Av. Instituto Politécnico Nacional 2508, Col. San Pedro Zacatenco, Del. GAM C.P. 07360 México D.F.
Teléfono 5747 3800 (Oficina 3368 Lab. 5120), Fax 5747 3754
Email: grafael@fisio.cinvestav.mx