Dr. Benjamín Florán Garduño

Investigador Cinvestav 3D
SNII Nivel III

  • Médico Cirujano (1983). FES-Zaragoza, UNAM
  • Maestría en Ciencias (1986). Depto. de Fisiología, Biofísica y Neurociencias. CINVESTAV
  • Doctor en Ciencias (1989). Depto. de Fisiología, Biofísica y Neurociencias. CINVESTAV
Sitio Web Institucional:
https://www.cinvestav.mx/zacatenco/fisiologia/conocimiento/directorio-de-investigacion/benjamin-floran-garduno
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Email

benjamin.floran@cinvestav.mx
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Teléfono

(+52) 55 5747 3800 +5137

Yasmín Ochoa García

Asistente Secretarial
yasmin.ochoa@cinvestav.mx
(+52) 55 5747 3800 +5188
Línea de Investigación:

Parkinson Experimental y Discinesias inducidas por L-dopa
    • Dopamina y receptores dopaminérgicos presinápticos
    • Sistema de Endocanabinoides
    • Adenosina y fármacos adenosinérgicos
    • Colaboraciones: Heteromerización de receptores acoplados a proteínas G
    • Terapéutica experimental: Terapia génica y Nanopartículas

Temas de Investigación:
    • Mecanismos de acción y toxicidad por l-dopa y fármacos antiparkinsónicos.
    • Interacciones dopamina-GABA-glutamato en los Ganglios Basales.
    • Interacciones dopamina con adenosina, opioides y endocanabinoides y sus vías de señalización. Dimerización

Metodología y Técnicas:
    • Liberación de neurotransmisores radioactivos
    • Western-Blot
    • Modelos Experimentales de Parkinson.
    • Estudios de Proximidad de ligandos
    • Microdiálisis “in vivo”. HPLC
    • Modelos conductuales de actividad motora
    • Estudios de receptores por “binding”
    • Registros electrofisiológicos en rebanadas

Dopamine D3 receptor modulates D2 receptor effects on cAMP and GABA release at striatopallidal terminals—Modulation by the Ca2+–Calmodulin–CaMKII system.

Flor Selene Villalobos-Escobedo, Rafael Jijón-Lorenzo, José Arturo Avalos-Fuentes, Francisco Paz-Bermúdez, Sergio Recillas-Morales, Israel Conde Rojas, Gerardo Leyva-Gómez, Hernán Cortés, Benjamín Florán.
European Journal of Neuroscience, 2024, Volume 59, Issue 7, pg. 1441-1459    https://doi.org/10.1111/ejn.16237

Modulation of D3R Splicing, Signaling, and Expression by D1R through PKA→PTB Phosphorylation.

Orlando Casados-Delgado, José Arturo Avalos-Fuentes, Manuel Lara-Lozano, Gisela Tovar-Medina, Carla Daniela Florán-Hernández, Karla Gisela Martínez-Nolasco, Hernán Cortes, Ricardo Felix, José Segovia and Benjamín Florán.
Biomedicines 2024, 12, 206. https://doi.org/10.3390/biomedicines12010206

Transfection of the BDNF Gene in the Surviving Dopamine Neurons in Conjunction with Continuous Administration of Pramipexole Restores Normal Motor Behavior in a Bilateral Rat Model of Parkinson’s Disease.

Alina Benítez-Castañeda, Verónica Anaya-Martínez, Armando de Jesús Espadas-Alvarez, Ana Luisa Gutierrez-Váldez, Luis Fernando Razgado-Hernández, Patricia Emmanuelle Reyna-Velazquez, Liz Quintero-Macias, Daniel Martínez-Fong, Benjamín Florán-Garduño, and Jorge Aceves.
Hindawi Parkinson´s Disease, Volume 2024, Article ID 3885451, 14 pages https://doi.org/10.1155/2024/3885451

Polymeric nanoparticles decorated with fragmented chitosan as modulation systems for neuronal drug uptake.

Hector Hernández-Parra, Hernán Cortés, Alejandra Romero-Montero, Fabiola V. Borbolla- Jiménez, Jonathan Javier Magaña, María Luisa Del Prado-Audelo, Benjamín Florán, Gerardo Leyva-Gómez.
El Sevier Carbohydrate Polymers 336 (2024) 122121 https://doi.org/10.1016/j.carbpol.2024.122121

Repositioning of drugs for Parkinson's disease and pharmaceutical nanotechnology tools for their optimization.

Hernandez-Parra H, Cortes H, Avalos-Fuentes JA, Del Prado-Audelo M, Floran B, Leyva-Gomez G, Sharifi-Rad J, Cho WC (2022).
J Nanobiotechnology 20:413. https://doi.org/10.1186/s12951-022-01612-5

Pharmacological targeting of G protein-coupled receptor heteromers.

Moreno E, Casajuana-Martin N, Coyle M, Campos BC, Galaj E, Del Torrent CL, Seyedian A, Rea W, et al. (2022).
Pharmacol Res 185:106476. https://doi.org/10.1016/j.phrs.2022.106476

Curcumin for Parkinson´s disease: potential therapeutic effects, molecular mechanisms, and nanoformulations to enhance its efficacy.

Hernán Cortés, Octavio D Reyes-Hernández, Maykel Gonzalez-Torres, Pablo A Vizcaino-Dorado, Maria Luisa Del Prado-Audelo, Sergio Alcalá-Alcalá, Javad Sharifi-Rad, Gabriela Figueroa-González, Manuel González-Del Carmen, Benjamín Florán, Gerardo Leyva-Gómez.
Cellular and Molecular Biology. (2021), 67 (1): 101-105.

D2 autoreceptor switches CB2 receptor effects on [3H]dopamine release in the striatum.

López-Ramirez, G., Sanchez-Zavaleta, R., Avalos-Fuentes, A., Sierra, J., Paz-Bermúdez, F., Leyva-Gómez, G., Segovia-Vila, J., Cortés, H., Florán, B.
Synapse. (2020), 74, 3:e22139.

Coexistence of D3R typical and atypical signaling in striatonigral neurons during dopaminergic denervation. Correlation with D3nf expression changes.

Campos, B., Avalos-Fuentes, A., Piña-Leyva, C., Sánchez-Zavaleta, R., Loya-Lopez, S., Rangel-Barajas, C., Leyva-Gómez, G., Cortes, H., Erlij, D., Floran B..
Synapse (2020), 74, 8 e22152.

D4R modulate inhibitory transmission in pallido-pallidal terminals and regulate motor behavior.

Conde-Rojas, I., Acosta, J., Caballero-Floran, RN., Jijón-Lorenzo, R., Recillas-Morales, S., Avalos-Fuentes, JA., Paz-Bermudez, F., Leyva-Gómez, G., Erlij, D., Cortes, H., Floran, B.
European Journal of Neuroscience. (2020), 52:4563–4585.

Presynaptic control of [3H] glutamate release by dopamine receptor subtypes in the rat substantia nigra. Central role of D1 and D3 receptors.

Briones-Lizardi LJ., Cortes., H., Avalos-Fuentes, JA., Paz-Bermúdez. FJ., Aceves, J., Erlij, D., Floran B.
Neuroscience. (2019), 406, 563-579.

Severity of dyskinesia and D3R signaling changes induced by L-DOPA treatment of hemiparkinsonian rats are features inherent to the treated subjects.

Albarrán-Bravo, S., Avalos-Fuentes, A., Cortes, H., Rodriguez-Sanchez, M., Leyva-García, N., Rangel-Barajas, C., Erlij, D., Floran, B.
 Biomolecules (2019), 9, 431.

Blockade of Intranigral and Systemic D3 Receptors Stimulates Motor Activity in the Rat Promoting a Reciprocal Interaction Among Glutamate, Dopamine, and GABA.

Rodriguez-Sanchez, M., Escartín-Perez, E., Cortes, H., Leyva-Gomez, G., Avalos-Fuentes, A., Paz-Bemudez, F., Loya-Lopez, S.I., Aceves, J., Erlij, D., Floran, B.
Biomolecules (2019), 9, 511.

Presynaptic cannabinoid CB2 receptors modulate [3H]-Glutamate release at subthalamo-nigral terminals of the rat.

Sanchez-Zavaleta, R, E., Cortes, F., Cortes, H., Paz-Bermudez, F., Erlij, D., Floran B.
Synapse. (2018), 72(11):e22061.

Presynaptic dopamine D2 receptors modulate [3H]GABA release at striatopallidal terminals via activation of PLC→IP3→Calcineurin and inhibition of AC→cAMP→PKA signaling cascades.

Jijón-Lorenzo, R., Caballero-Floran, I,H., Recillas-Morales, S., Cortés, H., Avalos-Fuentes, J.A., Paz-Bermudez, F.j., Erlij, D., Floran B.
Neuroscience. (2018), 372, 74-86.

Nanotechnology as Potential Tool for siRNA Delivery in Parkinson's Disease.

Cortés, H., Alcalá-Alcalá, S., Avalos-Fuentes, A., Mendoza-Muñoz, N., Quintanar-Guerrero, D., Leyva-Gómez, G., Floran, B.
Current Drug Targets. (2017), 18(16) 1866-1879.

Cannabinoid-induced depression of synaptic transmission is switched to stimulation when dopaminergic tone is increased in the globus pallidus of the rat.

Caballero-Florán, R.N., Conde Rojas, I., Oviedo-Chavez, A., Cortés-Calleja, H., Lopez-Santiago, L., Isom, L.I., Aceves, J., Erlij, D., Florán, B. 2016.
Neuropharmacology. 110:407-418.

Dopamine receptors and Neurodegeneration.

Rangel-Barajas, Claudia; Coronel, Israel; Florán, Benjamín.
Aging and Disease (2015), 6: (5): 349-368.

Nanoparticle technology for treatment of Parkinsonʹs disease: the role of surface phenomena in reaching the brain.

Gerardo Leyva-Gómez; Hernán Cortés; Jonathan J Magaña; Norberto Leyva-García; Benjamín Florán.
Review. Drug Discovery Today (2015), 20 (7) 824-837.

Dopaminergic denervation switches dopamine D3 receptor signaling and disrupts its Ca2+ dependent modulation by CAMKII and calmodulin in striatonigral projections of the rat.

Avalos-Fuentes, A., Albarrán-Bravo, S., Loya-Lopez, S., Cortés, H., Recillas-Morales, S., Magaña, J., Paz-Bermúdez, F., Rangel-Barajas, C., Aceves, J., Floran, B.
Neurobiology of Disease (2015), 74:336-346.

D3 dopamine receptors interact with dopamine D1 but not D4 receptors in the GABAergic terminals of the SNr of the rat.

Cruz-Trujillo, R., Avalos-Fuentes, J.A., Aceves, J., Erlij, D., Floran, B.
Neuropharmacology (2013), 67:370-378.

Presynaptic CAMKII modulates dopamine D3 receptor activation in striatonigral terminals of the rat brain in a Ca2+ dependent manner.

Avalos-Fuentes, J.A., Loya-Lopez, S., Rodriguez-Sanchéz, M., Recillas, S., Paz-Bermudez, F., Aceves, J., Erlij, D., Floran, B.
Neuropharmacology (2013), 71:273-281.

Dopamine D4 receptors in the Reticular Thalamic Nucleus and the Substantia Nigra reticulata of the rat decreases locomotor activity.

Erlij, D., Acosta, J., Escartín-Perez, R., Rojas-Márquez, M., Gonzalez-Hernandez, B., Aceves, J., Floran, B.
Neuropharmacology (2012), 62(2):1111-1118.

L-DOPA-induced dyskinesia in hemiparkinsonian rats is associated with up-regulation of adenylyl cyclase type V/VI and increased GABA release in the substantia nigra reticulata.

Rangel-Barajas, C.; Silva, I.; Lopez-Santiago, L. M.; Aceves, J.; Erlij, D., and Floran, B.
Neurobiol Dis. (2011), 41(1):51-61.

Estudiantes de Maestría

Ana Sinaí Pérez Hernández
Cotutor Dr. Marco Antonio Meraz (CINVESTAV)

anasinai.perez@cinvestav.mx

Mariana Torres Aguilar

mar.torres@cinvestav.mx

Estudiantes de Doctorado

Juan Antonio Aguilar Palomares
Cotutor Dr. Fatuel Tecuapetla (UNAM)

jose.aguilar@cinvestav.mx

Cecilia Sarai Castillo Chávez

sarai.castillo@cinvestav.mx

Regina Horta Victoria
Cotutor. Dra. Carolina Escobar (UNAM)

regina.horta@cinvestav.mx

Natalie Jiménez Barrios
Cotutor Dr. Rodolfo Delgado Lezama (CINVESTAV)

natalie.jimenez@cinvestav.mx

Sahian Alexandra Mejía Amador
Cotutor Dr. Rodolfo Delgado Lezama (CINVESTAV)

alexandra.mejia@cinvestav.mx

Personal

José Arturo Avalos Fuentes

Auxiliar de Investigación

javalos@cinvestav.mx

Francisco Javier Paz Bermúdez

Auxiliar de Investigación

francisco.paz@cinvestav.mx

José Luis Raya Tafolla

Técnico de Investigación

joraya@cinvestav.mx

Juan José Sierra Camacho

Técnico de Investigación

juanjose.sierra@cinvestav.mx